(thirteen). The sterol-binding and export purpose of yeast Pry proteins appears to be a conserved perform of customers in the CAP protein superfamily because expression on the human CAP protein CRISP2 complements the defect in sterol export of a yeast mutant lacking Pry operate, and purified CRISP2 binds sterols in vitro
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Fig. two. Cap domain and its purpose in TRPV1 perform. a Ribbon diagram of the sqTRPV1 extracellular part with subunits in various colour and the cap domain in blue. Charged residues are revealed as sticks. b Area view of exactly the same region coloured by electrostatic prospective with positively charged spots in blue, negatively charged in crimson and neutral in white. c Consultant traces from solitary-channel recording of spontaneous openings of wild-form sqTRPV1 and d606–628 mutant at various voltages in mobile-connected patch from HEK293T cells at home temperature. Black arrows point out the place with the baseline recent.
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